Archives des genetic toolbox - Bluetools project

Schematic overview of the study. The plasmid library was constructed by cloning a 200-nucleotide random sequence upstream of the thermostable reporter gene, superfolder citrine fluorescent protein (IFP). Plasmid DNA library was pooled from E. coli and transformed to T. thermophilus. Approximately 1500 T. thermophilus clones, arrayed on 96-well plates, were screened under UV light, and 53 fluorescent clones were selected for further characterization. The thermostable enzymes were transcriptionally fused to the IFP CDS. Plasmid DNAs were subsequently transformed into E. coli for comparative expression analysis.

Expanding the genetic toolbox for thermostable protein production

12 May 202625 March 2026

A new Open Access publication in Synthetic Biology journal presents an important step forward in enabling efficient protein production in the thermophilic bacterium Thermus thermophilus. The study addresses current limitations …